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Effect of isatin analogues on cyclooxygenase enzyme-II levels in LPS in RAW 264.7 macrophages. Cells were treated with different analogues for 1 h before LPS treatment. Following 24 h of incubation, level of Cox-2 were determined in cell supernatant using quantitative Sandwich ELISA technique. Celecoxib was used as a positive control drug. Data are expressed as Mean ± SD ( n = 3). Statistical analysis using Graph pad prism-one-way (multiple comparison test); * = p < 0.05, ** = p < 0.01, *** = p < 0.001 and **** = p < 0.0001 compared to the LPS-treated cells. ns: means no significant difference. LPS: lipopolysaccharide.

Journal: RSC Advances

Article Title: Design, synthesis, and anti-inflammatory evaluation of new isatin azo disperse dyes: computational studies, molecular docking, and printing performance on polyester fabrics

doi: 10.1039/d6ra01392a

Figure Lengend Snippet: Effect of isatin analogues on cyclooxygenase enzyme-II levels in LPS in RAW 264.7 macrophages. Cells were treated with different analogues for 1 h before LPS treatment. Following 24 h of incubation, level of Cox-2 were determined in cell supernatant using quantitative Sandwich ELISA technique. Celecoxib was used as a positive control drug. Data are expressed as Mean ± SD ( n = 3). Statistical analysis using Graph pad prism-one-way (multiple comparison test); * = p < 0.05, ** = p < 0.01, *** = p < 0.001 and **** = p < 0.0001 compared to the LPS-treated cells. ns: means no significant difference. LPS: lipopolysaccharide.

Article Snippet: The inflammatory mediator's interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and cyclooxygenase (COX-2) were assessed using a Sunlong Biotech Co., Ltd, China ELISA (Enzyme-Linked Immunosorbent Assay) kit.

Techniques: Analogues, Incubation, Sandwich ELISA, Positive Control, Comparison